E.G7-OVA(OVA/EL4)
CBPG0124
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| I. Background | |
| E.G7-OVA was derived in 1988 from the C57BL/6 (H-2 b) mouse lymphoma cell line EL4 (ATCC TIB-39). The EL4 cells were transfected by electroporation with the plasmid pAc-neo-OVA which carries a complete copy of chicken ovalbumin (OVA) mRNA and the neomycin (G418) resistance gene. | |
| II. Introduction | |
| Expressed gene: | OVA |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | RPMI-1640+10%FBS+0.05mM 2-mercaptoethanol+0.4 mg/ml G418 |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| III. Description of Host Cell Line | |
| Organism: | Mus musculus, mouse |
| Tissue: | T lymphocyte |
| Morphology: | Epithelial |
| Growth Properties: | suspension |
